大鼠脑立体定向手术校正的三种方法

摘要

Objective　The present paper introduces an integrated method for stereotaxic operation on the rat brain, using three different procedures for correcting the brain targeting site.Methods　Before the ordinary method for correcting the target site ,on the stereotaxic instrument the target site was exposured and the stereotaxic coordinates were corrected in fixed brain , and then unfixed brain, respectively.Results　As the application of three methods is set up,the defect of every method can be removed and the course of experiment can be shorten,so the efficiency of the tanget site in the rat brainon stereotaxic coordinates is improved.Conclusion　With fewer animals and fewer preliminary tests the brain target site can be corrected accurately.%目的介绍三种方法相结合的大鼠脑立体定位术程序书。方法在一般以切片观察确定靶位之前，先后分别将经过固定的死鼠、活鼠，固定在大鼠脑立体定位仪上，暴露靶点并校正靶坐标。结果三种方法结合运用后,克服了每种方法的缺点,缩短了预实验时间,提高了大鼠脑立体定向手术的效率。结论以少量的动物，经过较少次预实验，即能打准大鼠脑靶。