海藻酸钠纯度对微囊纤维化及囊内甲状旁腺组织功能的影响

摘要

Objective　To study the effect of alginate purity on implanted microcapsules and the function of human fetal parathyroid tissue in microcapsules.Methods　Microcapsules and microencapsulated human fetal parathyroid tissue produced with purified and unpurified alginate were xenografted into the peritoneal of mice which were divided into 4 groups. Transplanted empty microcapsules were established as control. Human parathyroid was measured to identify the secretory function of microcapsulated human parathyroid and the morphological changes of microcapsulated tissue were observed.Results　Two months after transplantation, there are significant differences in secreting function of microencapsulated parathyroid tissue between purified and unpurified groups(P＜0.05),(38.8±13.7) pg/ml and (1.2±0.7) pg/ml respectively. The retrieve rates of purified and unpurified alginate empty microcapsules are （72±18）% and (24±13）%，（65±21）% and 0 respectively after 1 and 2 months of transplantation.Conclusion　Microcapsules produced with purified alginate have good biocompatibility, the survival time of parathyroid tissue in the purified microcapsules was prolonged significantly.%目的研究海藻酸纯度对移植微囊及囊内人胚胎甲状旁腺组织功能的影响。方法将纯化及未纯化海藻酸微囊及微囊化人胚胎甲状旁腺组织分4组异种移植于小鼠腹腔内，设立空囊对照组。测定人甲状旁腺素数值，通过测定具有特异性的人甲状旁腺素，以明确微囊内甲状旁腺组织的分泌功能，组织学检测微囊形态。结果移植后2个月纯化组及未纯化组人甲状旁腺组织分泌功能有显著差异（P<0.05），分别为(38.8±13.7) pg/ml和(1.2±0.7) pg/ml，纯化及未纯化海藻酸钠制备空囊移植组，植入1个月时微囊冲洗回收率分别为（72±18）%和（24±13）%，植入2个月时为（65±21）%和0。结论纯化的海藻酸制备微囊具有良好的生物相容性，微囊内甲状旁腺组织发挥功能时间比未纯化海藻酸明显延长。