玫瑰黄链霉菌抗药性标记及其定殖和促生作用研究

摘要

选用腐霉利、多菌灵、甲基托布津、利福平、青霉素、链霉素、泰乐菌素、重铬酸钾等8种常用抗生素或杀菌剂,通过抗药性标记试验,建立了玫瑰黄链霉菌(Stremptomyces roseo flavus)Men-myco-93-63的抗药性标记,确定了重铬酸钾(0.06 g/L)/青霉素(0.2 g/L)为适合检测该菌株的标记药剂及浓度.通过获得的抗药性标记,结合菌落形态观察和16S rDNA序列分析,研究了Men-myco-93-63在番茄根际土壤中的定殖动态和对植株生长的影响.结果表明,该生防菌在番茄根际土壤中能够定殖,接菌15d后定殖量达到最高,为5.6×105 cfu/g,之后下降,到30 d后检测不到Men-myco-93-63菌株的存在.当使用Men-myco-93-63的固体发酵物与土壤以体积比1∶400混合时,对番茄苗期生长有促进作用.%In order to develop the antibiotic-resistance tag of Stremptomyces roseoflavus Men-myco-93-63, eight kinds of antibiotics or microbicides were tested, including procymidone, car-bendazim, thiophanate-methyl, rifabutin, penicillin, streptomycin, tylosin, potessium, and dichromate potassium. It was determined that potessium dichromate potassium with the concentration of 60 mg/L and penicillin with the concentration of 200 mg/L are the desirable antibiotic- resistance tag for the detection of Men-myco-93-63. Using developed tag, with the observation of colony morphology and the sequence analysis of 16S rDNA, colonizing dynamics of the strain around the tomato rhizosphere and their effects on the plants growth were evaluated. Results show that Men-myco-93-63 can colonize around the tomato rhizosphere. The amount of colonization reached the peak at the 15th day after inoculation, and then declined step by step. There was no Men-myco-93-63 detected 30 days after inoculation. Moreover, it was found thatMen-myco-93-63 showed growth -promoting effect on tomato seedling, if maturing the mixture of Men-myco-93-63 solid fermentation with soil in proportion of l:400.