糖尿病视网膜病变大鼠接受葡萄多酚干预后视网膜组织中细胞凋亡及氧化应激评估

摘要

目的：：研究葡萄多酚干预对糖尿病视网膜病变(DR)大鼠视网膜组织中细胞凋亡、氧化应激的影响。方法：选择 SPF 级雄性 SD 大鼠作为实验动物,分为对照组、糖尿病组、葡萄多酚组,采用链脲佐菌素腹腔注射的方式建立糖尿病大鼠模型,葡萄多酚组给予葡萄多酚灌胃处理。造模后60d,处死大鼠并收集视网膜组织,测定凋亡分子及氧化应激指标。结果：糖尿病组大鼠视网膜组织中 Bax、Caspase-3、c-fos、c-jun、ROS、MDA、8-OHdG、PARP、Cyclophilin D、Nrf-2、ARE、ERK、PI3K 的含量显著高于对照组,Bcl-2、CAT、SOD、GSH-Px、HO-1、NQO1的含量显著低于对照组；葡萄多酚组大鼠视网膜组织中Bax、Caspase-3、c-fos、c-jun、ROS、MDA、8-OHdG、PARP、Cyclophilin D 的含量显著低于糖尿病组, Bcl-2、CAT、SOD、GSH-Px、HO-1、NQO1、Nrf-2、ARE、ERK、PI3K 的含量显著高于糖尿病组。结论：葡萄多酚干预能够减轻糖尿病视网膜病变大鼠视网膜组织中的细胞凋亡以及氧化应激。%Objective:To study the effect of grape polyphenols intervention on apoptosis and oxidative stress in retina tissue of rats with diabetic retinopathy (DR).Methods:SPF male SD rats were selected as experimental animals and divided in-to control group,diabetes group and grape polyphenols group,intraperitoneal injection of streptozotocin was adopted to estab-lish diabetic rat models,and grape polyphenols group received intragastric administration of grape polyphenols.60 d after mod-el establishment,the rats were executed,and the retina tissue was collected to determine apoptosis molecules and oxidative stress indexes.Results:Bax,Caspase-3,c-fos,c-jun,ROS,MDA,8-OHdG,PARP,Cyclophilin D,Nrf-2,ARE,ERK and PI3K content in retina tissue of diabetes group were significantly higher than those of control group while Bcl-2,CAT,SOD, GSH-Px,HO-1 and NQO1 content were significantly lower than those of control group;Bax,Caspase-3,c-fos,c-jun,ROS, MDA,8-OHdG,PARP and Cyclophilin D content in retina tissue of grape polyphenols group were significantly lower than those of diabetes group while Bcl-2,CAT,SOD,GSH-Px,HO-1,NQO1,Nrf-2,ARE,ERK and PI3K content were signifi-cantly higher those of diabetes group.Conclusions:Grape polyphenols intervention can relieve the apoptosis and oxidative stress in retina tissue of rats with diabetic retinopathy.