In-Ceram 全瓷冠与金属烤瓷冠对成纤维细胞株 L929细胞活力及相关基因表达的影响

摘要

[ABSTRACT]Objective:To study the effect of In-Ceram all-ceramic crown and metal-ceramic crown on cell viability and related gene expression in fibroblast cell lines L929.Methods:Fibroblast cell lines L929 were cultured and treated with extract solution of In-Ceram all-ceramic crown,Ni-Cr alloy porcelain crown and Co-Cr alloy porcelain crown respectively,and then cell viability,serum cytokine contents as well as mRNA contents of Fas,FasL,Apo-1,mTOR and P70S6k in cells were detected. Results:Cell OD values of Ni-Cr alloy group and Co-Cr alloy group were significantly lower than that of negative control group;cell OD value of In-Ceram group was significantly higher than those of Ni-Cr alloy group and Co-Cr alloy group;TNF-α,IL-6 and IL-8 contents in cell culture medium of Ni-Cr alloy group and Co-Cr alloy group were significantly higher than those of neg-ative control group,and TNF-α,IL-6 and IL-8 contents in cell culture medium of In-Ceram group were significantly lower than those of Ni-Cr alloy group and Co-Cr alloy group and not different from those of negative control group;mRNA contents of Fas,FasL and Apo-1 in cells of Ni-Cr alloy group and Co-Cr alloy group were higher than those of negative control group,mR-NA contents of mTOR and P70S6k were lower than those of negative control group,mRNA contents of Fas,FasL and Apo-1 in cells of In-Ceram group were lower than those of Ni-Cr alloy group and Co-Cr alloy group and not different from those of negative control group,and mRNA contents of mTOR and P70S6k were higher than those of Ni-Cr alloy group and Co-Cr alloy group and not different from those of negative control group.Conclusion:In-Ceram all-ceramic crown has good histocompati-bility and will not affect cell viability as well as generation of inflammatory factors and expression of apoptosis and proliferation-related genes in fibroblast cell lines L929.%目的：：研究 In-Ceram 全瓷冠与金属烤瓷冠对成纤维细胞株 L929细胞活力及相关基因表达的影响。方法：培养成纤维细胞株 L929,分别用 In-Ceram 全瓷冠、镍铬合金烤瓷冠、钴铬合金烤瓷冠的浸提液处理,检测细胞活力、血清细胞因子含量以及细胞中 Fas、FasL、Apo-1、mTOR、P70S6k 的 mRNA 含量。结果：镍铬合金组、钴铬合金组细胞的 OD 值低于阴性对照组；In-Ceram 组细胞的 OD 值高于镍铬合金组、钴铬合金组；镍铬合金组、钴铬合金组细胞培养基中 TNF-α、IL-6、IL-8的含量明显高于阴性对照组,In-Ceram 组细胞培养基中 TNF-α、IL-6、IL-8的含量明显低于镍铬合金组、钴铬合金组且与阴性对照组无差异；镍铬合金组、钴铬合金组细胞中 Fas、FasL、Apo-1的 mRNA 含量高于阴性对照组, mTOR、P70S6k 的 mRNA 含量低于阴性对照组,In-Ceram 组细胞中 Fas、FasL、Apo-1的 mRNA 含量低于镍铬合金组、钴铬合金组且与阴性对照组无差异,mTOR、P70S6k 的 mRNA 含量高于镍铬合金组、钴铬合金组且与阴性对照组无差异。结论：In-Ceram 全瓷冠具有良好的组织相容性,不会影响成纤维细胞株 L929的细胞活力以及炎性因子的生成、凋亡和增殖相关基因的表达。