骨肉瘤中 SDF-1/CXCR4通路功能分析及外源性给予 SDF-1对骨肉瘤细胞的调节作用

摘要

[ABSTRACT]Objective:To study SDF-1/CXCR4 pathway function in osteosarcoma and the regulating effect of exogenous SDF-1 on osteosarcoma cells.Methods:Osteosarcoma tissue and adjacent tissue were collected to detect mRNA contents of SDF-1 (stromal cell-derived factor-1),CXCR4 (CXC chemokine receptor 4),Wnt,β-catenin,TCF and LEF;osteosarcoma MG63 cell lines were cultured,treated with SDF-1 and transfected with CXCR4-siRNA,and cell migration rate,number of in-vasive cells as well as mRNA contents of Wnt,β-catenin,TCF,LEF,c-myc,cyclinD1,MMP2,MMP9 and VEGF were de-tected.Results:The mRNA contents of SDF-1,CXCR4,Wnt,β-catenin,TCF and LEF in osteosarcoma tissue were signifi-cantly higher than those in adjacent tissue;cell migration rate,number of invasive cells as well as mRNA contents of Wnt,β-catenin,TCF,LEF,c-myc,cyclinD1,MMP2,MMP9 and VEGF in SDF-1 treatment group were higher than those in control group;cell migration rate,number of invasive cells as well as mRNA contents of Wnt,β-catenin,TCF,LEF,c-myc,cy-clinD1,MMP2,MMP9 and VEGF in SDF-1 +CXCR4 interference group were lower than those in SDF-1 treatment group. Conclusions:SDF-1/CXCR4 pathway function in osteosarcoma is enhanced,and exogenous SDF-1 can promote osteosarcoma cell migration and invasion and activate Wnt/β-catenin pathway through CXCR4.%目的：：研究骨肉瘤中 SDF-1/CXCR4通路功能及外源性给予 SDF-1对骨肉瘤细胞的调节作用。方法：收集骨肉瘤组织和瘤旁组织,测定 SDF-1、CXCR4、Wnt、β-catenin、TCF、LEF 的 mRNA 含量；培养骨肉瘤 MG63细胞株,用 SDF-1处理、转染 CXCR4-siRNA,测定细胞迁移率、侵袭数目以及 Wnt、β-catenin、TCF、LEF、c-myc、cyclinD1、MMP2、MMP9、VEGF 的 mRNA 含量。结果：骨肉瘤组织中 SDF-1、CXCR4、Wnt、β-catenin、TCF、LEF 的 mRNA 含量均明显高于瘤旁组织；SDF-1处理组细胞的迁移率、侵袭数目以及 Wnt、β-catenin、TCF、LEF、c-myc、cyclinD1、MMP2、MMP9、VEGF 的 mRNA 含量高于对照组；SDF-1+CXCR4干扰组细胞的迁移率、侵袭数目以及 Wnt、β-catenin、TCF、LEF、c-myc、cy-clinD1、MMP2、MMP9、VEGF 的 mRNA 含量低于 SDF-1处理组。结论：骨肉瘤中 SDF-1/CXCR4通路功能增强,外源性给予 SDF-1能够通过 CXCR4促进骨肉瘤细胞迁移、侵袭并激活 Wnt/β-catenin 通路。