超声引导下穿刺所得卵巢癌组织中LSD1、PARP1与细胞增殖、上皮间质转化的相关性

摘要

目的:研究超声引导下穿刺所得卵巢癌组织中LSD1、PARP1与细胞增殖、上皮间质转化的相关性.方法:收集在医院超声引导下穿刺所得的卵巢癌组织和卵巢良性病变组织,检测卵巢癌组织和卵巢良性组织中LSD1、PARP1的mRNA表达量以及细胞增殖分子、上皮间质转化分子的蛋白含量.结果:卵巢癌组织中LSD1、PARP1的mRNA表达量显著高于卵巢良性组织;卵巢癌组织中P21、P27、E-cadherin的蛋白含量均显著低于卵巢良性组织,CyclinD1、E2F、Twist1、Snail、Slug、N-cadherin的蛋白含量均显著高于卵巢良性组织;LSD1高表达的卵巢癌组织中P21、P27的蛋白含量均显著低于LSD1低表达的卵巢癌组织,CyclinD1、E2F的蛋白含量均显著高于LSD1低表达的卵巢癌组织;PARP1高表达的卵巢癌组织中Twist1、Snail、Slug、N-cadherin的蛋白含量均显著高于PARP1低表达的卵巢癌组织,E-cadherin的蛋白含量均显著低于PA RP1低表达的卵巢癌组织.结论:卵巢癌组织中高表达的LSD1、PARP1能够促进癌细胞的增殖及上皮间质转化.%Objective:To study the correlation of LSD1 and PARP1 with cell proliferation and epithelial-mesenchymal transition in ovarian cancer tissue from ultrasound-guided puncture .Methods:The ovarian cancer and ovarian benign lesion tis-sue from ultrasound-guided puncture in Pangang Group General Hospital in Panzhihua between May 2014 and March 2017 were collected to detect the mRNA expression of LSD1 and PARP1 as well as the protein levels of cell proliferation molecules and epithelial-mesenchymal transition molecules in them .Results:LSD1 and PARP1 mRNA expression in ovarian cancer tissue were significantly higher than those in benign ovarian tissue ;P21 ,P27 and E-cadherin protein levels in ovarian cancer tissue were significantly lower than those in benign ovarian tissue while CyclinD 1 ,E2F ,Twist1 ,Snail ,Slug and N-cadherin protein levels were significantly higher those in benign ovarian tissue ;P21 and P27 protein levels in the ovarian cancer tissue with high LSD1 expression were significantly lower than those in the ovarian cancer tissue with low LSD 1 expression while CyclinD1 and E2F protein levels were significantly higher than those in the ovarian cancer tissue with low LSD 1 expression;Twist1 ,Snail , Slug and N-cadherin protein levels in the ovarian cancer tissue with high PARP 1 expression were significantly higher than those in the ovarian cancer tissue with low PARP1 expression while E-cadherin protein level was significantly lower than that in the ovarian cancer tissue with low PARP1 expression .Conclusion:The LSD1 and PARP1 highly expressed in ovarian cancer tissue can promote the proliferation and epithelial-mesenchymal transition of cancer cells .