子宫平滑肌中大电导钙激活钾通道α、β亚基表达量与宫缩乏力性产后出血的相关性

摘要

目的:研究子宫平滑肌中大电导钙激活钾通道(BKCa)α、β亚基表达量与宫缩乏力性产后出血的相关性.方法:选择2015年3月~2017年5月期间在攀枝花市妇幼保健院接受剖宫产且发生宫缩乏力性产后出血的产妇作为研究的出血组,另取同期在攀枝花市妇幼保健院进行剖宫产且无产后出血的产妇作为对照组.剖宫产中取子宫肌组织适量,测定子宫肌中BKCa的α、β亚基的表达量、收缩相关蛋白的含量以及子宫肌的收缩特征参数.结果:出血组产妇的子宫肌组织中BKCa的α亚基、β亚基的m RN A表达量及蛋白表达量均显著高于对照组;出血组产妇子宫肌组织的收缩幅度、收缩频率、收缩活动力以及子宫肌组织中O T R、COX2、CX43、HSP27的含量均显著低于对照组;出血组产妇子宫肌组织中BKCa的α亚基、β亚基表达量与收缩幅度、收缩频率、收缩活动力以及O T R、COX2、CX43、HSP27的含量均呈负相关关系.结论:子宫平滑肌中BKCa的高表达能够降低子宫肌收缩力、减少收缩相关蛋白的含量,与宫缩乏力性产后出血的发生密切相关.%Objective:To study the correlation of large conductance Ca2+ activated K+ channel (BKCa)αandβ subunit expression in uterine smooth muscle with the postpartum hemorrhage induced by uterine inertia .Methods:The puerperae who underwent cesarean section and had postpartum hemorrhage induced by uterine inertia in Panzhihua Women and Children Health Hospital between March 2015 and May 2017 were selected as the hemorrhage group of the study ,and the puerperae who underwent cesarean section and were without postpartum hemorrhage in Panzhihua Women and Children Health Hospital during the same period were selected as the control group .Proper amount of uterine muscle tissue was collected during the cesarean section to measure the expression of BKCa α and β subunits and the levels of contraction-related proteins in uterine muscle as well as the contraction characteristic parameters of the uterine muscle .Results:The mRNA expression and protein expression of BKCa α and β subunits in uterine muscle tissue of hemorrhage group were significantly higher than those of control group;the contraction amplitude ,contraction frequency and contraction activity of uterine muscle tissue as well as the OTR , COX2 ,CX43 and HSP27 levels in uterine muscle tissue of hemorrhage group were significantly lower than those of control group;the BKCa αandβsubunit expression in uterine muscle tissue of hemorrhage group were negatively correlated with the contraction amplitude ,contraction frequency and contraction activity as well as the OTR ,COX2 ,CX43 and HSP27 levels . Conclusion:The high expression of BKCa in uterine smooth muscle can reduce the uterine muscle contractility and decrease the levels of contraction-related proteins ,and it is closely related to the occurrence of postpartum hemorrhage induced by uterine inertia .