Effect of inhibiting miR-155 expression on proliferation and migration of airway smooth muscle cell in patients with COPD

摘要

Objective:To investigate the effect of inhibiting miR-155 expression on the proliferation and migration of airway smooth muscle cells(ASMCs)in patients with chronic obstructive pulmonary disease(COPD).Methods:ASMCs were isolated and cultured from 8 patients with COPD(observation group)and 3 patients with benign lung cancer without COPD(control group).The ASMCs were transfected with miR-155 suppression expression plasmid(to detect the expression of miR-155;flow cytometry was used to detect the cell cycler of cell clones;Transwell was used to detect cell migration and invasion;Enzyme-linked immunosorbent aanti-miR-155)and the negative contre;clone formation experiment was used to detect the numbol plasmid(anti-miR-NC),and blank control group was set.Real-time quantitative PCR(RT-qPCR)was usedssay(ELISA)method was used to detect tumor necrosis factor-α(TNF-α),interleukin-6(IL-6)level.Results:The expression level of miR-155 in ASMCs of observation group was significantly higher than that in the control group(P<0.05).The miR-155 expression level in inhibited miR-155 expression group was significantly lower,compared with the negative control group and the blank group(P<0.05).In the inhibited miR-155 expression group,the proportion of G0-G1 phase cells was increased,the proportion of S phase cells was decreased,the number of cell clones,migration,and the number of invasive cells were decreased,and the levels of TNF-αand IL-6 were increased(P<0.05).Conclusions:Inhibiting the expression of miR-155 can inhibit the proliferation and migration of airway smooth muscle cells in COPD patients,and inhibit the release of proinflammatory factors.