Effects of raspberry rhizome decoction on expression of C-myc and Cath-D in tumor tissues of S180 tumor-bearing mice

摘要

Objective: To investigate the effects of RRD on the expression of nuclear protein regulatory genes (C-myc) and cathepsin D (Cath-D) in tumor tissues of S180 tumor-bearing mice, and to analyze the anti-tumor effect of RRD. Methods: Forty clean ICR healthy Kunming (KM) mice were randomly divided into four groups: model group, traditional Chinese medicine group (RRD), cyclophosphamide group (CTX) and combined drug group (RRD + CTX). Ten mice in each group were subcutaneously inoculated with S180 cell suspension at the axillary position to establish the model. After 24 h, the model group was given orally 0.02 mL/g/d with normal saline; RRD group was given orally 0.4 mL/20 g/d with RRD; CTX group was given orally 0.4 mL/20 g/d with normal saline and intraperitoneally 20 mg/kg/d with CTX; RRD+CTX group was given orally 0.4 mL/20 g/d with RRD and intraperitoneally 20 mg/kg/d with CTX once a day for 10 d. The expression of C-myc and Cath-D in tumor tissues was detected by immunohistochemistry. Results: RRD could significantly decrease the expression of C-myc and Cath-D in the tumor tissues of S180 tumor-bearing mice, and there was a significant difference between RRD and model group. Conclusion: The inhibitory effect of RRD on S180 tumor-bearing mice may be related to the inhibition of the expression of C-myc and Cath-D related proteins in tumor proliferation, invasion and metastasis.