MEKK3 and NF-κB expression levels in cervical intraepithelial neoplasia tissue and their correlation with high-risk HPV infection

摘要

Objective:To study the MEKK3 and NF-κB expression levels in cervical intraepithelial neoplasia (CIN) tissue and their correlation with high-risk human papilloma virus (HPV) infection.Methods:A total of 500 patients with cervical intraepithelial neoplasia were selected as CIN group, 450 chronic cervicitis patients with matched general data were selected as control group, cervical tissue was collected, immunohistochemical method was used to detect the protein expression levels of mitogen-activated protein kinase/ERK kinase kinase 3 (MEKK3) and nuclear factors-κB (NF-κB), fluorescence quantitative PCR method was used to detect the mRNA expression levels ofMEKK3, NF-κB, JAK2, STAT3, p16INK4a, p27kip1 andp53kip3.Results:The protein expression levels and mRNA expression levels of MEKK3 and NF-κB in cervical tissue of CIN group were significantly higher than those of control group; the protein expression levels and mRNA expression levels ofMEKK3 and NF-κB in cervical tissue with high-risk HPV infection (+) were significantly higher than those in cervical tissue with high-risk HPV infection (-); mRNA levels ofJAK2andSTAT3in CIN cervical tissue with positive expression of MEKK3 and NF-κB were significantly higher than those in CIN cervical tissue with negative expression of MEKK3 and NF-κB, and mRNA levels ofp16INK4a, p27kip1 andp53kip3were significantly lower than those in CIN cervical tissue with negative expression of MEKK3 and NF-κB.Conclusions:High-risk HPV infection can increase the expression levels of MEKK3 and NF-κB in cervical intraepithelial neoplasia tissue, and then down-regulate the expression of tumor suppressor genesp16INK4a, p27kip1 andp53kip3 through the JAK2/STAT3 signaling pathway.