Effect of hTERC and FOXP3 gene magnification in condyloma acuminate lesion on the immune response and cell apoptosis

摘要

Objective:To study the effect of hTERC and FOXP3 gene amplification in condyloma acuminate lesions on the immune response and cell apoptosis.Methods:The condyloma acuminate lesions diagnosed between May 2014 and October 2016 and the normal skin tissue from circumcision during the same period were collected to extract RNA and then determine hTERC, FOXP3 and apoptosis-related gene mRNA amplification, and after protein extraction, the protein levels of immune response-related cytokines were determined.Results:hTERC, FOXP3, Livin and Survivin gene mRNA amplification as well as IL-4 and IL-10 protein levels in condyloma acuminatum lesions were significantly higher than those in normal skin tissue while TRAIL, Caspase-3 and PDCD4 mRNA amplification as well as IL-2 and TNF-α protein levels were significantly lower than those in normal skin tissue; IL-2 and TNF-α protein levels in condyloma acuminatum lesions with high FOXP3 mRNA expression were significantly lower than those in condyloma acuminatum lesions with low FOXP3 mRNA expression while IL-4 and IL-10 protein levels were significantly higher than those in condyloma acuminatum lesions with low FOXP3 mRNA expression; Livin and Survivin mRNA amplification in condyloma acuminatum lesions with high hTERC mRNA expression were significantly higher than those in condyloma acuminatum lesions with low hTERC mRNA expression while TRAIL, Caspase-3 and PDCD4 mRNA amplification were significantly lower than those in condyloma acuminatum lesions with low hTERC mRNA expression.Conclusion:Highly expressed hTERC and FOXP3 genes in condyloma acuminatum lesions can inhibit apoptosis and inhibit antiviral immune response respectively.