HPLC测定苦牛大力根部和甜牛大力不同部位中芒柄花素和高丽槐素的含量

摘要

Objective:To establish an HPLC method for the quantification of Formononetin and Maackiain in root of Radix Millettia Championi and different parts of Radix Millettia Speciosa.Methods:HPLC column was Welchrom-C18 (4.6 mm× 250 mm,5μm) with acetonitrile-water (37.5:62.5) as mobile phase.The flow rate was set to 1.0 mL/min,the column temperature was 28 ℃ and the wavelength of Formononetin and Maackiain for detection were 248 nm and 309 nm,respectively.Results:There was a good linear relationship between the injection volume and the peak area (r=0.999,9),when the volume of Formononetin and Maackiain were within the range of 0.0068-0.1428 μg and 0.152-3.800 μg,respectively.The recovery rates of Formononetin and Maackiain were 97.06 ％ and 104.62 ％,respectively.The contents of Maackiain and Formononet were higher in the root of Radix Millettia Championi than those in the root of Radix Millettia Speciosa,and was roots＞rhizomes＞stems＞leaves of Radix Millettia Speciosa.Conclusion:The HPLC method established here could be used in the quality control of Formononetin and Maackiain.Radix Millettia Championi was more valuable for exploitation than Radix Millettia Speciosa.Different parts of Radix Millettia Speciosa existed Formononetin and Maackiain,providing a theoretical basis for its utilization.%目的:建立HPLC同时测定芒柄花素和高丽槐素含量的方法,对比苦牛大力根部和甜牛大力不同部位中芒柄花素和高丽槐素的含量.方法:Welchrom-C18色谱柱(4.6 mm×250 mm,5μm),流动相为乙腈-水(37.5∶ 62.5),流速1.0 mL/min,柱温28℃,芒柄花素和高丽槐素的检测波长分别为248 nm、309 nm,进样量为15 μL.结果:芒柄花素进样量为0.006 8～0.142 8 μg,高丽槐素进样量为0.152～3.800 μg,与峰面积值呈良好的线性关系(r=0.999 9),芒柄花素和高丽槐素的平均回收率分别为97.06％、104.62％.芒柄花素和高丽槐素分布趋势:苦牛大力根部＞甜牛大力根部;甜牛大力根部＞根茎部＞茎＞叶.结论:该HPLC条件可用于苦牛大力中芒柄花素和高丽槐素的含量测定;苦牛大力根部芒柄花素和高丽槐素的含量高于甜牛大力根部,提示苦牛大力具有较大的药用开发价值;甜牛大力各部位均含有芒柄花素和高丽槐素且含量不同,本研究结果可为甜牛大力的综合利用提供一定的理论依据.