应用60Co产生的γ射线照射人肝癌细胞系SMMC-7721,以克隆形成法测定经照射后的细胞存活率,用化学诱导剂Calyculin-A.诱导的早熟染色体凝集技术研究染色体损伤.结果显示G2期细胞内的染色单体和等点染色单体断裂数与照射剂量之间存在着线性相关性,染色单体断裂数与细胞存活率之间存在较好的线性相关性.表明辐射诱导的染色单体断裂可以作为预测SMMC-7721细胞内在辐射敏感性的指标,也可为临床诊断和治疗肝癌提供依据.%To investigate the irradiation response of SMMC-7721 hepatoma cells, a human hepatoma cell line SMMC-7721 was exposed to 60Co γ rays. The cell survival fraction was described by colony assay; G2 chromosome aberration was detected by premature chromosome condensation technique. After irradiation, the cell survival fraction decreased with the dose increase in linearly quadratic. G2 chromatid breaks and isochromatid breaks linearly increased with the dose. This study implied a good correlation between intrinsic radiosensitivity and G2 chromosome aberration of the human hepatoma cell line SMMC-7721.
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