电纺丝聚乳酸纤维支架的制备及其细胞相容性研究

摘要

Electrospun poly（D,L,lactic acid）（PDLLA） superfine fiber has become one of the hot spots regarding scaffolds materials in tissue engineering,due to its high porosity,tunable pore size and structure,as well as good biocompatibility.In this study,we investigated the factors including concentration,solution feeding rate,applied voltage and drying methods that affecting the morphology of PDLLA fibrous scaffolds.The optimum process parameters were determined： solution concentration was 0.20g/mL,solution feeding rate was 0.8mL/h,applied voltage was 12kV.After lyophilization,uniformly distributed PDLLA fibers with diameters of 500-900nm were obtained.In vitro cell morphology observation showed that PDLLA fibrous scaffolds having good cytocompatibility.%静电纺丝法制备的聚（D,L-乳酸）超细纤维具有孔隙率高、孔径/结构可调、生物相容性良好等特点,已成为组织工程支架材料研究的热点。探讨了纺丝溶液浓度、流量、电压、干燥方式等影响聚（D,L-乳酸）纤维支架形貌的因素,确定了最佳工艺参数为纺丝溶液浓度为0.20g/mL,流速为0.8mL/h,电压为12kV,经真空冷冻干燥,可获得分布比较均匀,直径大多为500～900nm的聚乳酸纤维。体外细胞形貌观察表明聚（D,L-乳酸）纤维支架具有良好的细胞相容性。