磷脂胆碱仿生修饰聚乳酸及其生物学评价

摘要

通过自由基聚合反应将马来酸酐接枝到聚（D,L-乳酸）（PDLLA）侧链上,然后通过引入的活性反应基团马来酸酐与甘油磷脂胆碱反应,实现磷脂胆碱对聚乳酸的整体仿生修饰。采用红外光谱和核磁共振碳谱对磷脂胆碱修饰聚乳酸进行了表征。生物降解实验表明,该功能化聚乳酸具有明显抑制酸致降解作用。体外细胞形貌观察证实磷脂胆碱修饰的聚乳酸具有良好的细胞相容性。同时,蛋白吸附和血小板粘附实验表明,该聚合物具有良好的血液相容性。该改性聚乳酸可望用作组织工程支架及血液接触类植入体。%The maleic anhydride is grafted onto the side-chain of poly（D, L-lactic acid） （PDLLA）by free radical polymerization firstly. Secondly the maleic anhydride is reacted with glycerophosphorylcholine（GPC）. Thus the phospholipid choline bionic chemical modification with PDLLA can be got. It was characterized by fourier trans- form infrared spectroscopy （FT-IR）and^13 C-NMR. Biodegradation test showed that this functional poly（D, L- lactic acid） could significantly inhibit＇the acid induced degradation of the PDLLA. In vitro cell morphology ob- servation confirmed that GPC modified PDLLA was cytocompatible. Meanwhile, Protein adsorption test and platelet adhesion test revealed that the GPC modified PDLLA was also hemocompatible. These results suggest that GPC modified PDLLA could be an excellent biocompatible candidate for the development of tissue engineer- ing scaffold and blood contacting implants.