石墨烯/蚕丝蛋白复合膜的构建、表征与生物相容性评估

摘要

Graphene oxide (GO) was prepared from natural flake graphite by chemical oxidation method.GO aqueous suspension was blended with different weight ratio 1%, 5%, 10% degumming silk fibroin (SF) solution to fabricate GO/SF composite films via vacuum filtering.Then the GO/SF films were covalent cross-linked by EDC/NHS and chemically reduced by hydroiodic acid.Finally, the "brick and mud" layered structure composite films with excellent mechanical properties were obtained.Properties of rGO/SF such as contact angel, mechanical properties, morphology, structure and in vivo biocompatibility were systematic characterization.The results revealed that hydrophilicity, mechanical properties and biocompatibility of composite films were improved with the increase of SF content and chemical crosslinking effect.The results of this study demonstrate that this composite film has potential applicationas biomaterial scaffolds in tissue engineering and regenerative medicine.%以天然鳞状石墨为原料,采用化学氧化法合成氧化石墨烯,与脱胶后的蚕丝丝素蛋白按比例混合后通过真空抽滤构建1%,5%和10%(质量分数)不同丝素含量的氧化石墨烯/丝素蛋白复合薄膜,并通过EDC/NHS进行化学共价键交联,最后采用氢碘酸对复合薄膜进行还原进而得到具有优良力学特性的"砖-泥"层状结构的无机/有机复合薄膜.研究分析了薄膜的亲疏水性、力学性能、形貌、结构与体内生物相容性.研究结果表明,丝素组分的添加与共价键化学交联作用不仅改善了复合薄膜的亲水性及力学性能,也增加了复合薄膜的体内生物相容性.这种基于石墨烯/丝素蛋白复合薄膜有望作为生物材料支架应用于组织工程与再生医学领域.