烟熏肉制品中多环芳烃测定的不确定度评定

摘要

目的：评定用气相色谱-质谱法测定烟熏肉制品中多环芳烃残留量的不确定度。方法采用微波萃取法提取烟熏肉制品中的多环芳烃，经凝胶渗透色谱仪净化处理，用气相色谱-质谱法对烟熏肉制品中多环芳烃的残留量进行分析，建立数学模型，对测量结果的各不确定度来源进行分析和量化。结果本方法的不确定度主要来自于标准溶液的配制及标准曲线的建立。当烟熏肉制品中多环芳烃的含量为4.92~8.76μg/kg时，其扩展不确定度为0.37~0.78μg/kg (k=2)。结论该评定方法可用于气相色谱-质谱法对烟熏肉制品中多环芳烃残留量测定的不确定度评定。%Objective To evaluate the uncertainty in determination of polycyclic aromatic hydrocarbons (PAHs) in smoke-dried meat products by gas chromatography-mass spectrometry (GC-MS).Methods The PAHs in sample were extracted by microwave extraction, purified by gel permeation chromatography (GPC), and then analyzed by GC-MS. The mathematical model was established based on the above analysis to analyze and quantify the sources of uncertainty of the measurement results.Results The major uncertainty sources in this method were preparation of standard solutions and establishment of curve calibrations. When the PAHs content in smoke-dried meat products ranged from 4.92μg/kg to 8.76μg/kg, the extended uncertainty was 0.37~0.78μg/kg (k=2).Conclusion The method is suitable for the evaluation of uncertainty of PAHs in smoke-dried meat products determined by GC-MS.