食用油过氧化值快速检测试剂盒的研制及其性能评价

摘要

Objective To develop a new type of rapid detection kit for the determination of peroxide value in oils .MethodsThe befitting alternative solvents and the best ratio of reagents were explored. The standard color card was established according to national standard method (GB 5009.37-2003) principle, and color reactions can be carried out through the reaction carrier, and then the peroxide value of oils was tested by the naked eye. In order to verify the accuracy and stability of this kit, the peroxide value of oil stored at room temperature for 3 months were obtained and compared by kit’s method and national standard method.ResultsEthanol and isooctane (70:30,V:V) which were safe and low-toxic were selected as mixed solvent of kit experiment. It was rapid to determine peroxide value through color reaction in easily accessible carrier. The test range was 0.5~20 meq/kg. This kit maintained high accuracy and stability during 3 months.ConclusionThis kit can be applied for the rapid screening of peroxide value in oil, which has broad market prospects.%目的：研制一种新型的食用油中过氧化值快速检测的试剂盒。方法探索合适的替代溶剂以及最佳的试剂配比；依据GB 5009.37-2003原理制作标准比色卡,在反应载体上进行显色反应,通过目视比色快速判定待测油脂的过氧化值。在食用油常温存放的3个月内,同时采用试剂盒方法和国标方法检测其过氧化值,通过比较验证试剂盒的准确性和稳定性。结果选择安全低毒的乙醇：异辛烷(70：30, V：V)作为试剂盒实验的混合溶剂,在配置的简单载体上可快速进行显色反应,可检测的过氧化值区间范围为0.5~20 meq/kg。试剂盒在常温下存放的3个月内都能保持较好的准确度和稳定性。结论该试剂盒可用于食用油中过氧化值的快速筛查,具有广泛的市场应用前景。