Objective To establish a method for the determination of tadalafil and other 2 analogues (amino tadalafil and dimethyl tadalafil) in alcoholic beverage by ultra performance liquid chromatography (UPLC).Methods Agilent Zorbax SB-C18 column (2.1 mm×50 mm,1.8 μtm) was used with a mobile phase consisting of acetonitrile and 0.01 mol/L ammonium acetate (pH=3.4) by gradient elution at a flow rate of 0.3 mL/min.The illegally added antifatigue drugs were detected by a photodiode array detector.The detection wavelength was set at 290 nm.Results The linearity ranges of amino tadalafil,dimethyl tadalafil and tadalafil were 0.6～120,0.493～98.6,and 0.56～112 mg/L,respectively.The limits of determination were 0.06,0.06,and 0.05 ng and the precisions of the method (RSDs) were 0.92％,1.1％ and 0.68％ (n=6),respectively.The average recoveries were 101.8％,101.8％ and 101.7％ (n=6)and the RSDs were 1.51％,0.87％ and 0.64％ (n=6),respectively.Conclusion The established method has the advantages of simple sample pre-treatment,rapid detection,satisfactory recovery and good reproducibility,which is suitable fbr the determination of illegally added antifatigue drugs in alcoholic beverage.%目的 建立超高效液相色谱法测定酒饮料中他达拉非和2种类似物的分析方法.方法 采用Agillent Zorbax SB-C18 (2.1 mm×50 mm,1.8μttm)色谱柱,二极管阵列检测器,检测波长为290 nm,流速为0.3 mL/min,流动相A为0.1％乙酸铵溶液(pH=3.4),流动相B为乙腈,按梯度进行洗脱.结果 氨基他达拉非、去甲基他达拉非、他达拉非分别在0.6～120、0.493～98.6、0.56～112 mg/L范围内呈现良好的线性关系,检出限分别是0.06、0.06、0.05 ng,方法精密度分别为0.92％、11％、0.68％(n=6),平均回收率分别为101.8％、101.8％、101.7％,RSD分别为1.51％、0.87％、0.64％(n=6).结论 该方法前处理简单、分析时间短、灵敏度高、重现性好,可用于抗疲劳类化学药物在酒精饮料中非法添加的检测.
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