肉桂双瓣卷蛾为害对樟树叶片挥发物的影响

摘要

采用顶空固相微萃取(SPME)及气相色谱-质谱联用仪(GC-MS)技术分析肉桂双瓣卷蛾为害前后的樟树叶片挥发物变化.结果表明,健康和虫伤的樟树叶片中均含有26种相同的挥发物成份,但是肉桂双瓣卷蛾为害后挥发物的相对含量发生显著变化.肉桂双瓣卷蛾危害后,叶片中的黄樟素含量显著增高,健康叶片中黄樟素仅占0.6％,而虫伤叶片中为2.7％;但是反-水合倍半香桧烯、顺-橙花叔醇、反-橙花叔醇及α-红没药烯含量均显著降低,健康叶中分别含1.9％、4.5％、11.6％和1.2％,而虫伤叶片中分别占0.6％、2.1％、7.0％和0.7％.挥发物组分含量变化可能在调控害虫取食、天敌对猎物的搜寻等行为过程中发挥作用.本研究为探索植物挥发物对害虫及天敌行为的影响奠定基础,为了解寄主-昆虫-天敌三层营养关系的相互作用、利用天然活性化合物防治害虫提供理论依据.%Headspace solid phase micro extraction (SPME) and gas chromatography-mass spectrometry (GC-MS) technology were used to campare volatiles between normal and Polylopha cassiicola damaged leaves of Cinnamomum camphora.Results showed that normal and P.cassiicola injured leaves contained the same 26 volatiles,but relatively content of some volatiles were altered after P.cassiicola damage.Specifically,the content of safrole was significantly increased after insect damage.The relative content of safrole was only 0.6％ in normal leaves,while it was increased up to 2.7％ in P.cassiicola damaged leaves.Neverrtheless,the relative contents of cis-Sesquisabinene hydrate,cis-nerolidol,anti-nerolidol and α-bisabolene were significantly reduced after P.cassiicola damage,they were decreased from 1.9％,4.5％,11.6％ and 1.2％ in normal leaves to 0.6％,2.1％,7.0％ and 0.7％ in P.cassiicola damaged leaves,respectively.The alteration of content of volatiles might play a key role in regulating the insect feeding and prey searching behavior of natural enemy.This study lay the foundation for exploring the impact of plant volatiles on the behaviour of insect pests and their natural enemy,providing theoretical basis for understanding the three trophic relationships interaction among host plant,insect pest and natural enemy,as well as the use of natural active compounds for the control of insect pests.