In order to explore the non-destructive methods for sex determination of the live pupae of Hepinlus altaicola Wang (Lepidoptera:Hepialidae),the body parameters of live pupae (including thorax width and length,abdomen width and length,body length,and body weight) of each sex were measured and data were analyzed using Student's t-test by GraphPad Prism 5 software.Meanwhile,the other external morphology characteristics on the sternites of male and female pupae were observed and compared to find the non-destructive methods for sex determination of the live pupae.The pupae were divided into two groups according to the morphological differences of the 8th and 9th abdominal sternites.The two groups of pupae were kept under laboratory conditions and were allowed to develop to the adult stage.The confirmation of the sex was carried out by inspection of the adult genitalia or pupal gonad.Though the female pupae are larger than male pupae,there were significant differences between males and females in the thorax width and length,abdomen width and length,body length and weight.But the ranges of above-mentioned body parameters in male and female showed broad overlap.So these differences between male and female pupae can not be used as reliable sex-related external markings.However,it was found that male and female pupae could be conclusively distinguished based on differences of the ventral parts on the 8th and 9th abdominal sternites.In female pupa,one extension of 9th abdominal sternite exists in the middle of the 8th abdominal sternite,the genital pore is located on the 8th abdominal sternite,and no round lump on each side of genital pore is existed;but in male,the extension is not seen,and the lump is obviously visible,meanwhile,the genital pore is located on the 8th abdominal sternite.In addition,the distance between the genital pore and the genus is longer in female than in male.Based on above mentioned characteristics,we explored non-destructive methods for sex determination of the live pupae of H.altaicola,and the discriminant accuracy of each method was 100％.These methods based on the differences of the sexual characteristics existed on the 8th and 9th abdominal sternites are non-destructive,accurate,reliable,simple and quickly learned.%通过观察阿尔泰蝠蛾雌雄蛹的外部形态结构,并测量其体征和体重数据,寻找可用于鉴别蛹性别的无损方法;再借助于解剖等方法鉴别蛹的确切性别,检测鉴别方法的准确性.结果显示,雌雄蛹在体征和体重数据的分布范围上有重叠,不能籍此区分其雌雄性.然而,雄蛹腹面第8腹节和第9腹节间分节明显,第9腹节未向第8腹节纵伸,生殖孔位于第9腹节上,生殖孔两侧各有一球形突起,生殖孔前缘与肛门前缘间的距离短于肛门的长度;雌蛹不具备以上特征.基于这些特征开发的鉴别阿尔泰蝠蛾蛹雌雄的方法,鉴别活蛹的准确率均为100％(n=180).该方法对活蛹无损伤、准确、可靠、简便易学.
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