青藤碱对大鼠小肠移植后缺血/再灌注损伤的保护作用

摘要

Objective:To observe the effect of sinomenine on ischemia / reperfusion injury in rats following intestinal transplantation,and study its protective mechanism.Methods:40 receptor SD rats after small bowel transplantation were randomly divided into ischemia/reperfusion group and sinomenine intervention group (n =20),the other 16 SD rats as the control group.After operation,the sinomenine intervention group was treated with 10％ sinomenine [10 mL/(kg,d)] for 14 days,and the control group and the ischemia /reperfusion group received Sodium Chloride injection [10 mL/(kg.d)].CCR5 and the expression of β-arrestin 2 and β-arrestin 2-mRNA in intestinal mucosa tissue were detected with EnVision two step method;NO,NOS,T-SOD and MDA in intestinal tissue were detected with ELISA Kit.Results:Sinomenine could reduce NO and MDA in intestinal tissue of the rat,increase T-SOD.In the invention group,there was the high expression of β-arrestin 2 and β-arrestin 2-mRNA in intestinal mucosa tissue and the low expression of CCRS.Compared with the ischemia/reperfusion group and the same group before intervention,the difference was statistically significant (P ＜ 0.05).Conclusion:Sinomenine can inhibit lipid peroxidation after intestinal transplantation and modulate the local immune response after intestinal transplantation by affecting the expression of beta-arrestin 2 and CCRS.It has protective effects on ischemia / reperfusion injury after allograft transplantation in rats.%目的 观察青藤碱对同种异体大鼠小肠移植后缺血/再灌注损伤的影响,研究其保护机制.方法 将小肠移植后的40只受体SD大鼠随机分为缺血/再灌注组和青藤碱干预组,另取16只SD大鼠为对照组.术后青藤碱干预组用10％青藤碱[10 mL/(kg·d)]灌胃干预性治疗14d,对照组及缺血/再灌注组按[10 mL/(kg.d)]灌0.9％氯化钠注射液.采用EnVision二步法检测小肠黏膜组织CC趋化因子受体5(CCR5)和β-抑制蛋白2(β-arrestin 2)和β-arrestin 2-mRNA的表达,ELISA试剂盒检测小肠组织一氧化氮(NO)、一氧化氮合成酶(NOS)、总超氧化物歧化酶(T-SOD)和丙二醛(MDA).结果 青藤碱可降低大鼠小肠组织NO和MDA,提高T-SOD,且青藤碱干预组小肠黏膜β-arrestin 2和β-arrestin 2-mRNA高表达,CCR5低表达,与缺血/再灌注组和同组干预前比较,差异有统计学意义(P＜0.05).结论 青藤碱可明显抑制小肠移植后脂质过氧化反应、并通过影响β-arrestin 2和CCR5的表达来调节小肠移植后局部免疫反应,对同种异体大鼠小肠移植后缺血/再灌注损伤有保护作用.