Objective To investigate the difference of RANTES (regulated on activation normal T-cell expressed and secreted) expression between asthmatic mice and control mice after respiratory syncytial virus (RSV) infection in murine model of asthma. Methods A murine model of asthma was made by sensitizing BALB/c mice with ovalbumin (OVA). After infecting the asthmatic mice with RSV, the concentrations of RANTES and interferon-gamma in bronchoalveolar lavage fluid (BALF) were measured. In addition, the number of inflammatory cells in BALF after the viral infection was examined. Results Although both asthmatic mice and control mice showed increased RANTES expression after RSV infection, the difference between the two groups was not significant. When asthmatic mice were compared before and after RSV infection, the number of eosinophil was not changed but the number of neutrophil was increased. The interferon-gamma level was not raised by RSV infection in both asthmatic and control groups. Conclusions This study found that RSV infection does not lead to enhanced RANTES expression in asthmatic mice as compared to control mice. This finding implies that RSV infection induces RANTES expression regardless of asthma.%目的 研究体外培养的新生大鼠皮质神经元缺氧不同时间后缺氧诱导因子(HIF)1α及血管内皮生长因子(VEGF)的表达规律及意义.方法 体外培养的新生大鼠皮质神经元于厌氧培养箱中缺氧0、2、4、8 h,用Western blot及real time PCR检测缺氧不同时间后神经元HIF-1α及VEGF mRNA及蛋白的表达.结果 常氧培养的神经元中几乎不表达HIF-1α,VEGF表达较低;缺氧2 h后,神经元中HIF-1α及VEGF mRNA表达均显著增加;缺氧4 h后两者mRNA及蛋白表达最高;缺氧8 h后表达降低.结论 缺氧增加神经元中HIF-1α及VEGF的表达,随着缺氧时间的增加呈先升高后降低的趋势.
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