目的：研究慢性运动性疲劳状态下，大鼠中枢神经系统各种单胺类递质的变化规律。方法应用慢性运动性中枢疲劳大鼠模型，取材大鼠额叶皮质，采用高效液相色谱电化学检测的方法，对实验大鼠额叶皮质组织5-羟色胺、肾上腺素、去甲肾上腺素及多巴胺的含量进行定量分析。结果与正常饲养大鼠比较，疲劳大鼠在持续训练条件下发生运动性中枢疲劳后，其额叶皮质5-羟色胺含量明显升高，同时多巴胺的含量明显下降，并在统计学出现非常显著性差异（ P <0.01）；而同样属于单胺类神经递质的肾上腺素和去甲肾上腺素的水平则没有出现统计学差异。结论在中枢性疲劳发生时，中枢神经系统内两种重要神经递质5-羟色胺和多巴胺的水平同时发生了改变，且这两种变化的方向均为抑制性趋势，这也说明该模型大鼠形成了中枢疲劳状态，这种状态的形成引起了脑内递质水平调节机制的变化。%Objective The paper aims to study the regulatory changes of monoamines transmitters in the central nerve system of rats under the state of chronic exercise - induced fatigue. Methods The rat model of chronic exercise - induced central fatigue was used in this study. The frontal cortex of the rats was removed. The contents of 5 - HT,epinephrine,norepinephrine and dopamine in frontal cortex were quantitatively ana-lyzed by high performance liquid chromatography(HPLC). Results It was found that compared with control group,there was a significant in-crease of frontal cortex 5 - HT in rats with exercise - induced central fatigue after constant training and there was,at the same time,a significant decrease of dopamine( P < 0. 01). However,there was no significant change of the levels of epinephrine and norepinephrine which both belong to monoamine neurotransmitter. Conclusion With central fatigue,the levels of 5 - HT and dopamine,the two important neurotransmitters in cen-tral nerve system,have significant changes simultaneously. And both of the changes have a direct inhibitory trend,which explains that the rats are under the state of central fatigue and this state causes the change of regulatory mechanism of the transmitter in the brain.
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