高效液相色谱法同时检测植物甾醇与植物甾醇酯

摘要

Simultaneous detection method of phytosterols and phytosterol esters was studied by reversed-phase HPLC.Acetone and acetonitrile(3∶1)was used as mobile phase,12 compounds of phytosterols and phytosterol esters could be separated completely under the conditions of flow rate 1.0 mL/min,column temperature 30 ℃ and isocratic elution.Analysis time was only 25 minutes in this method,and there was no sample pretreatment.Limit of detection ranged from 5.1 to 41.1 μg/mL,and limit of quantitative ranged from 17.0 to 137.0 μg/mL,and recovery rates ranged from 93.81％ to 111.98％.Sterol linoleate and sterol oleate in the concentration range of 0.01 ～1.0 mg/mL showed a linear correlation.Linearity range of sterol linoleate and sterol oleate was 0.1 ～ 1.0 mg/mL.All the regression coefficients of standard curves were over 0.998.This method could be used in simultaneous detection of different sterol and sterol ester products,and worthy of promotion.%采用反相高效液相色谱,对同时检测植物甾醇和甾醇酯的分析方法进行了研究.通过检测条件优化,植物甾醇和甾醇酯的12种不同组分在以丙酮和乙腈(3:1)为流动相,柱温30℃,流动相流速1.0ml/min,等度洗脱的条件下,达到最好分离效果.此方法不需要样品的前处理,分析时间25 min,检出限为5.1 ～1.1μg/mL,定量限为17.0 ～ 137.0 μg/mL,回收率93.81％ ～111.98％.植物甾醇与甾醇乙酸酯在0.01 ～ 1.0 mg/mL的浓度范围内呈线性相关,甾醇亚油酸酯与甾醇油酸酯在0.1～1.0 mg/mL范围内具有良好的线性关系,标准曲线相关系数均超过0.998.该方法可应用于多种甾醇和甾醇酯产品的同时检测.