地塞米松对人牙周膜细胞白细胞介素6和白细胞介素8表达的调节作用

摘要

目的 探讨地塞米松(Dex)对人牙周膜细胞(hPDLCs)中白细胞介素(IL)6和IL?8表达的调节作用.方法 Dex或牙龈卟啉单胞菌(P.g)-脂多糖(LPS)分6组处理hPDLCs:10-9 mol/L Dex组,10-6 mol/L Dex组,10μg/mL P.g?LPS组,10-9 mol/L Dex+10μg/mL P.g?LPS组,10-6 mol/L Dex+10μg/mL P.g?LPS组,0.1%无水乙醇(对照组).分别用ELISA法和实时定量PCR法检测IL?6、IL?8蛋白和mRNA表达水平.结果 24 h和48 h时,10-9 mol/L Dex组和10-6 mol/L Dex组IL?6和IL?8蛋白表达水平显著低于对照组(P<0.05).48 h时10-9 mol/L Dex组和10-6 mol/L Dex组hPDLCs IL-6 mRNA水平显著低于对照组(P<0.05);而10-6 mol/L Dex组IL?8 mRNA的表达水平显著高于对照组(P<0.05).24 h和48 h时10-9 mol/L Dex+10μg/mL P.g?LPS组和10-6 mol/L Dex+10μg/mL P.g?LPS组IL?6蛋白和mRNA表达水平显著低于10μg/mL P.g?LPS组(P<0.05).24 h时10-9 mol/L Dex+10μg/mL P.g?LPS组和10-6 mol/L Dex+10μg/mL P.g?LPS组IL?8蛋白表达水平显著低于10μg/mL P.g?LPS组(P<0.05),但48 h未见统计学差异.48 h时,10-6 mol/L Dex+10μg/mL P.g?LPS组IL?8 mRNA水平显著高于10μg/mL P.g?LPS组(P<0.05).结论 Dex可以显著抑制hPDLCs固有的和P.g?LPS诱导产生的IL?6的表达,但是Dex对hPDLCs IL?8的表达具有复杂的调节作用,需注意高浓度Dex长时间作用时对hPDLCs IL?8表达的促进作用.%Objective To explore the effect of dexamethasone(Dex)treatment on the expression of interleukin(IL)?6 and IL?8 in human peri?odontal ligament cells(hPDLCs). Methods hPDLCs were subjected to one of the following treatments for 24 h or 48 h:10-9 mol/L Dex;10-6 mol/L Dex;10μg/mL Porphyromonas gingivalis(P. g)?lipopolysaccharide(LPS);10-9 mol/L Dex+10μg/mL P. g?LPS;10-6 mol/L Dex+10μg/mL P. g?LPS;or 0.1%absolute ethyl alcohol(control). Protein and mRNA expression was detected using ELISA and real?time PCR ,respectively. Results At 24 h and at 48 h,IL?6 and IL?8 protein expression in the 10-9 mol/L Dex?and 10-6 mol/L Dex?treated groups was significantly lower than that in the control group(P<0.05). At 48 h,IL?6 mRNA expression in the 10-9 mol/L Dex?and 10-6 mol/L Dex?treated groups was signifi?cantly lower than that in the control group(P<0.05),while IL?8 mRNA expression in the 10-6 mol/L Dex?treated group was significantly higher than that in the control group(P<0.05). At 24 h and at 48 h,IL?6 protein and mRNA expression in the 10-9 mol/L Dex+10μg/mL P. g?LPS?treated group and the 10-6 mol/L Dex+10μg/mL P. g?LPS?treated group was significantly lower than that in the 10μg/mL P. g?LPS?treated group (P<0.05). At 24 h,IL?8 protein expression in the 10-9 mol/L Dex+10μg/mL P. g?LPS?treated group and the 10-6 mol/L Dex+10μg/mL P. g?LPS?treated group was significantly lower than that in the 10μg/mL P. g?LPS?treated group(P<0.05),while no such significant difference exist?ed at 48 h. At 48 h,IL?8 mRNA expression in the 10-6 mol/L Dex+10μg/mL P. g?LPS?treated group was significantly higher than that in the 10μg/mL P. g?LPS?treated group(P<0.05). Conclusion Dex inhibits the innate and P. g?LPS?induced expression of IL?6 in hPDLCs. However, Dex exerts profound effects on IL?8 expression,and treatment with high doses of Dex may promote IL?8 expression over an extended period.