首页> 中文期刊> 《中南林业科技大学学报》 >芫花粗提物对鳞翅目幼虫消化酶和解毒酶的影响

芫花粗提物对鳞翅目幼虫消化酶和解毒酶的影响

         

摘要

卫矛尺蠖和菜粉蝶幼虫取食涂有芫花乙醇粗提物的叶子后,1~2 d内即可使消化酶及 谷胱甘肽酶活力指数显著上升,此后回落到对照或显著低于对照水平.电镜观察表明,24 h 后,中肠细胞粗糙内质网上的蛋白体数量增加,着色加深,显示这些部位的功能更趋活跃; 48 h后,细胞内线粒体内嵴开始模糊,体积肿大;72 h后,部分线粒体成为空壳.以上事实 为中毒昆虫的生理变化提供了亚细胞水平的依据.%24~48 hours after the larva have eaten leaves c ontaminated with Yuanhua(Daph ne genkwa) alchohol crude extract (YAE),the activities of digestion enzymes (A mylae s,Esterases,lnvertase,Proteinase) and GSTs are enhanced;thereafter the activiti es decline to or become lower than the level of control.Electron microscope pic tures show that the number of micro-protein-particles on RER is increased and mit ochondria becomes larger in the period; after then the inner ridges of mitochond ria become in distinct,and the inner contents of mitochondria are depleted. These phenomena ex plain the dynamics of the digestion enzymes, GSTs and the lost weigh t of insects in the posterior period.

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