Immobilization of laccase on BC membrane and use for decolorization of reactive brilliant blue KN-R was investigated. Kinetics of the degrading of brilliant blue and influence of different factors on decolorization of the brilliant blue was studied. The results showed that degradation of the brilliant blue by immobilized laccase matched with two order kinetic equation. Adsorption of the brilliant blue on BC membrane matched with Freundlich isotherm. When the enzyme activity was 84.9 U, the decolorization efficiency was 73.5%. The best decolorization efficiency was 81.1% when the dye concentration was 50 mg/L. Decolorization of the brilliant blue by immobilized enzyme preferred acidic conditions, and the optimum pH was 5, with which the decolorization efficiency reached as high as 95.2%. Compared with free enzyme, the degradation rate of immobilized enzyme was increased by 2 times, and the reaction rate was increased by 3 times. The decolorization efficiency of the brilliant blue was reduced to 12.2% after 5 times repeated decolorization by the same immobilized enzyme. The absorption peaks in the UV visible absorption spectra of the decolorized solution of reactive brilliant blue KN-R almost disappeared completely, which indicated that dye was degraded.%采用细菌纤维素(BC)膜为载体固定化漆酶,对活性艳蓝KN-R脱色,研究了艳蓝降解的动力学和不同因素对该染料脱色的影响.结果表明,脱色后的艳蓝溶液的紫外可见吸收峰几乎完全消失,说明该染料发生降解脱色;固定化酶对艳蓝的降解符合二级动力学方程;BC 膜对艳蓝的吸附符合Freundlich吸附等温线.当固定化酶的活力为84.9 U时,脱色率最大为73.5%;染料浓度为50 mg/L时,脱色效果较好,为81.1%;固定化酶对艳蓝的脱色在偏酸性条件下更好,最适宜的pH为5,此时的脱色率为95.2%;与游离酶相比,固定化酶的降解率提高了2倍,反应速率提高了3倍;在重复脱色5次后,脱色率降为12.2%.
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