Detecting katG Drug Resistant Genetic Mutation among pneumoconiosis patients complicated with tuberculosis in Mycobacterium tuberculosis L-forms by PCR-SSCP

摘要

Objective:To study the relationship between mutation in the katG gene and drug resistance of INH in Mycobacterium tuberculosis L-forms among patients of pneumoconiosis complicated with pulmonary tuberculosis, and to explore the clinical application of PCR-SSCP. Methods:A total of 52 clinical isolated strains of Mycobacterium tuberculosis L-forms were collected. Mutation in the katG genes was detected by PCR-SSCP and traditional antimicrobial susceptibility test(AST). Results: The results by AST showed that there were 40 persisters in 52 clinical isolated strains. The drug resistant rate was 76.92%(40/52), and the gene mutation rate of katG was 57.70%(30/52)by PCR-SSCP, the difference was no quite significance(χ2 = 2.8507, P > 0.05). The coincidence rate of two methods was 75.00%(30/40). Conclusion: The detectionrate of katG resistant strains in Mycobacterium tuberculosis L-forms was high by PCR-SSCP. The combined application of PCR-SSCP and traditional antimicrobial susceptibility test can improve the detecting rate.