Microalgal diet is the key factor in shellfish growth and breeding.To gain a thorough knowledge of the effect of microalgal diets on the growth of early life stages of bivalves, liquid chromatograph-mass spectrometer (LC-MS) analytical method about high-sensitivity and high-definition of structure information for microalgae and shellfish was established, and a breeding mode with selective feeding of different microalgae bait according to different shellfish at different development stages was also built.Many unicellular microalgae were isolated and purified from natural water.Eventually, two microalgal species (3 strains) with high efficient bait effects on shellfish juvenile at different stages were screened out based on the lipid composition and the actual effect of lipid nutrients bait, which filled the gap of feed supply for shellfish breeding in rainy season.Meanwhile, high-throughput and continuous supply of microalgal diets for larger juveniles had been realized.%饵料微藻的种类和营养组成影响着贝类的营养、生理与繁殖.为了深入了解微藻饵料对双壳贝类繁育的影响,建立了完整的针对微藻和贝类脂类结构的高灵敏度、结构信息高清晰的液质联用技术,并以此为基础建立了针对不同发育阶段的不同贝类,选择性投喂不同微藻饵料的苗种繁育模式;同时,通过从天然海域分离纯化单细胞微藻,根据脂类营养成分分析并结合实际饵料效果,筛选出2种(3株)适宜贝苗不同阶段摄食的新型高效饵料微藻,填补了贝类苗种培育行业阴雨季饵料供给的缺口,实现了大规格稚贝饵料的高通量连续供给.
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