Tγδ17和Tγδ22细胞在健康成人外周血中的比例

摘要

目的:观察健康成人外周血中αβT细胞和γδT细胞中辅助性T细胞17(TH17)样细胞和TH22样细胞的比例.方法:采集18名正常人外周血用荧光抗体染色后用流式细胞仪检测αβT细胞、γδT细胞中白细胞介素-22(IL-22)、IL-17和γ干扰素(IFN-γ)表达细胞的百分比.结果:将正常人外周新鲜全血中CD3+ T细胞分成γδT 细胞和αβT细胞两群.TH1样细胞在αβT细胞中比例为14.31%,明显低于在γδT细胞中的61.07%(P0.05).结论:在γδT细胞中存在IL-17和IL-22均阳性的Tγδ17细胞和仅产生IL-22的Tγδ22细胞,但比αβT细胞中的TH17样细胞和TH22样细胞少.%Objective:To observe the proportions of TH17-like cells and TH22-like cells in αβT cells and γδT cells in healthy adult peripheral blood. Methods: Eighteen healthy adult peripheral blood samples were stained with fluorescent antibody and the percentages of interleukin-22,interleukin-17 and IFN-γ-producing cells in αβT cells and γδT cells were detected by flow cytometry. Results:The CD3 + T cells in the whole blood cells were divided into two groups:αβT cells and γδT cells. The proportions of TH1 -like cells,TH17-like cells and TH22-like cells in αβT cells were 14. 31% ,1. 16% and 1. 06% ,respectively. While the proportions of TH1-like cells, TH17-like cells and TH22-like cells in γδT cells were 61. 07% ,0. 27% and 0. 91% ,respectively. The proportion of TH1-like cells in αβT cells was lower than in γδT cells,and the proportion of TH17-like cells in αβT cells was higher than in γδT cells ( P 0. 05 ) . Conclusions: There exists IL-17 and IL-22 double-positive Tγδ17 cell subpopulation and IL-22 single-positive Tγδ22 cell subpopulation in γδT cells. But the proportions are lower than those in αβT cells.