鼻咽癌细胞株中乳腺癌转移抑制基因表达的研究

摘要

Objective:To detect the expressions of breast cancer metastasis suppressor 1 ( BRMS1 ) gene in different human nasopharyngeal carcinoma(NPC) cell lines. Methods:The expressions of BRMS1 protein and mRNA in SUNE-1-5-8F,CNE-1,CNE-2Z,SUNE-1 and SUNE-1-6-10B of NPC cell lines were detected by immunohistochemical method and RT-PCR,respectively. Results:The positive expressions of BRMS1 protein in CNE-1,CNE-2Z,SUNE-1,SUNE-1-5-8F and SUNE-1-6-10B cell lines were identified. Except the SUNE-1-5-8F cell line,the mRNA expressions of BRMS1 in other four NPC cell lines were positive. The expression levels of BRMS1 mRNA in CNE-1,CNE-2Zand SUNE-1 cell lines were significantly higher than that in SUNE-1-6-10B cell line. Conclusions:The expression of BRMS1 is correlated to the metastatic ability of cell line, which may be an important marker in identifying the metastasis of NPC.%目的：：检测乳腺癌转移抑制(BRMS1)基因在不同转移习性的人鼻咽癌细胞株中的表达情况。方法：应用免疫组织化学法和逆转录聚合酶链式反应法在mRNA和蛋白2方面检测人鼻咽癌细胞株高分化磷癌、低分化磷癌、低分化细胞株、高成瘤高转移性细胞株、低成瘤低转移性细胞株中BRMS1的表达。结果：5种细胞株中BRMS1的蛋白表达皆为阳性,在不同转移习性的细胞株中其表达强度明显不同。4种细胞株中扩增出BRMS1 mRNA,而BRMS1 mRNA在高转移性细胞株中未扩增出,高分化鳞癌、低分化鳞癌、低分化细胞株BRMS1 mRNA表达水平均显著高于低成瘤低转移性细胞株(P<0.01)。结论：BRMS1的表达与细胞株的转移能力有明显相关性,表明BRMS1可能作为鼻咽癌细胞转移的重要指标。