基于抗痴呆类药成分西伯利亚远志糖A5、西伯利亚远志糖A6和远志(咄)酮Ⅲ的开心散质量控制方法研究

摘要

Objective To found a quality control method of Kaixin San based on anti-dementia drug-like components including sibricose A5, sibricose A6 and xanthone III, and compare and analyze the extractive quantity of 3 effective components extracted mixedly from whole formula of Kaixin San and Yuanzhi (Radix Polygalae). Methods The procedure of HPLC-DAD was performed on the chromatographic column of Waters XbridgeTM Shield C18 (4.6 mm ×250 mm, 5 μm) at 30 ℃ , and the mobile phase was acetonitrile-0.1% phosphoric acid solution in gradient elution. The flow velocity was 1.0 mL/min and the detection wavelength was 330 run for sibricose A5 and sibricose A6 nm, and 254 nm for xanthone M. Results Sibricose A5 showed a good linear relationship at a range from 0.043 μg to 1. 085 μg, sibricose A, from 0.090 μg to 2. 250 μg, and xanthone HI, from 0.008 μg to 0. 234 μg. The average recovery was, respectively, 96. 39% , 97. 37% and 99. 66%. The mixture extraction of whole formula of Kaixin San and extraction of Yuanzhi (Radix Polygalae) had influence on the extractive quantity of 3 effective components. Conclusion The method was simple and reliable, which can be used for the quantitative determination of sibricose A5, sibricose A6 and xanthone IH as well as quantity control of Kaixin San.%目的 建立基于抗痴呆类药成分西伯利亚远志糖A5、西伯利亚远志糖A6和远志(山)酮Ⅲ的开心散质量控制方法,并运用此方法对开心散水提物和远志水提物的3个有效成分的提取量进行比较分析.方法 采用HPLC-DAD分析方法,Waters Xbridge TM shield C18柱(4.6mm×250mm,5μm),以乙腈-0.1％磷酸水溶液为流动相梯度洗脱,流速1 mL/min.检测波长西伯利亚远志糖A5、西伯利亚远志糖A6为330 nm,远志(咄)酮Ⅲ为254 nm,柱温30℃.结果 西伯利亚远志糖A5、西伯利亚远志糖A6和远志(咄)酮Ⅲ分别在0.043 ～1.085 μg、0.090～2.250 μg、0.008 ～0.234μg范围内线性关系良好；平均回收率分别为96.39％、97.37％和99.66％.开心散水提物和远志水提物对3个有效成分的提取量有影响.结论 3批样品含量测定结果表明,该方法简便、准确,可用于开心散提取物中类药成分西伯利亚远志糖A5、西伯利亚远志糖A6和远志(咄)酮Ⅲ的含量测定,亦可作为开心散定量控制方法之一.