肝癌组织中 SRC-3的表达及其与肝癌临床病理特征的关系

摘要

目的：探讨肝癌组织中类固醇受体共激活因子-3（SRC-3）表达及其与肝癌临床病理特征的关系。方法采用免疫组织化学染色和 RT-PCR 检测80例肝癌组织和癌旁组织中 SRC-3蛋白和 mRNA 表达水平。结果肝癌组织中 SRC-3蛋白阳性表达率为56.25%，明显高于癌旁组织的8.75%，差异有统计学意义（P ﹤0.05）。58例肝癌组织中 SRC-3 mRNA 呈高水平转录，癌旁组织仅有12例出现高水平转录，肝癌组织中 SRC-3 mRNA 转录水平较癌旁组织明显升高（P ﹤0.05）。合并乙型肝炎病毒（HBV）感染肝癌患者 SRC-3蛋白表达阳性率为63.49%，明显高于未合并 HBV 感染肝癌患者的29.41%，差异有统计学意义（ P ﹤0.05）；甲胎蛋白（AFP）﹤400 ng·mL -1肝癌患者 SRC-3蛋白表达阳性率为76.19%，明显高于 AFP≥400 ng·mL -1肝癌患者的49.15%，差异有统计学意义（P ﹤0.05）；高分化肝癌患者 SRC-3蛋白表达阳性率为88.24%，明显高于中、低分化患者的47.62%，差异有统计学意义（P ﹤0.05）。结论 SRC-3的高表达与肝癌的疾病进展关系密切，可能成为潜在的肝癌分子标志物或治疗靶点。%Objective To investigate the expression of steroid receptor coactivator-3(SRC-3)in the hepatocellu-lar carcinoma tissues and its relationship with clinicopathological features. Methods immunohistochemical staining and RT-PCR were used to detect SRC-3 protein and mRNA expression levels in the 80 cases of hepatocellular carci-noma and paraneoplastic tissues. Results The positive expression rate of SRC-3 protein in hepatocellular carcinoma tissues was 56. 25% ,which was significantly higher than that(8. 75% )in the paraneoplastic tissues(P ﹤ 0. 05). SRC-3 mRNA was high level transcription in the 58 cases of hepatocellular carcinoma,and was 12 cases in the para-neoplastic tissues,SRC-3 mRNA transcription level in the hepatocellular carcinoma was significantly increased than the paraneoplastic tissue(P ﹤ 0. 05). The positive rate of SRC-3 protein expression in the patients with hepatitis B virus(HBV)infection was 63. 49% ,which was significantly higher than that(29. 41% )of patients with non HBV infection,the difference was statistically significant(P ﹤ 0. 05);the positive rate of SRC-3 protein expression in the patients with alpha-fetoprotein(AFP) ﹤ 400 ng·mL - 1 was 76. 19% ,significantly higher than that(49. 15% )in the patients with AFP≥400 ng·mL - 1 ,the difference was statistically significant(P ﹤ 0. 05);the positive rate of SRC-3 protein expression in the patients with high differentiated hepatocellular carcinoma was 88. 24% ,significantly higher than that(47. 62% )in the patients with inmiddle and low differentiated hepatocellular carcinoma,the differ-ence was statistically significant(P ﹤ 0. 05). Conclusion SRC-3 high expression is related with the canceration and progression of hepatocellular carcinoma,and may become a potential molecular marker or therapeutic target.