亚硒酸钠影响无机汞/甲基汞对青海弧菌Q67的毒性∗

摘要

Objective:Mercury is a global pollutant and is listed as priority pollutants by many international organizationsbecause of its persist-ence in the environment, the bioaccumulation and toxicity in the food chain. The toxic effect of inorganic mercury( HgCl2 ) , methyl-mercury( Me-HgCl) and selenium(Na2SeO3)alone and their combinationon Vibrio qinghaiensis sp. -Q67(Q67) was studied. Methods:Q67 in the luminescent bacteria was used as the subject, combined with high-throughput microplate detection technology, with EC50 ( half maximal effective concentra-tion) as a measure index of the toxicity. Different exposure concentrations of inorganic mercury, methyl-mercury and selenium were used to deter-mine the luminous inhibition rate. Results:The EC50 value of inorganic mercury(0. 29687 mg/L) was significantly higher than that of methylmercu-ry(0. 08964 mg/L). When the combined mercury and selenium was exposed at the low concentrations of mercury, the luminous inhibition rate of Q67 has decreased. Conclusion:The toxicity of methylmercury to Q67 is stronger than that of inorganic mercury. Acertain antagonism is produced by sodium selenite with inorganic mercury and methylmercury at low concentrations, with that of selenium to methylmercury more significant.%目的：：研究无机汞(HgCl2)、甲基汞(MeHgCl)和硒(Na2SeO3)单独及联合暴露下对青海弧菌Q67(Vibrio qing-haiensis sp.-Q67)毒性效应。方法：以发光菌中的青海弧菌Q67为受试生物，结合微孔板高通量检测技术，借用半数最大效应浓度( EC50)作为衡量毒性强弱的指标。用不同浓度的无机汞、甲基汞以及硒对其暴露，测定其发光抑制率。结果：无机汞的EC50值(0.29687mg/L)高于甲基汞的EC50值(0.08964mg/L)。硒汞联合暴露时，汞在低浓度的条件下，青海弧菌Q67的发光抑制率都有所降低。结论：甲基汞对青海弧菌Q67的毒性作用明显强于无机汞。亚硒酸钠与低浓度条件下的无机汞和甲基汞都产生了一定的拮抗作用，硒对甲基汞产生的拮抗作用更为明显。