金银花中绿原酸和木犀草苷的同时提取及测定研究

摘要

Objective： To optimalize the extracting conditions of chlorogenic acid and galuteolin from Flos lonicerae, and develop one method for the content determination of chlorogenic acid and galuteohn. Methods： Through designing a L9 （34） orthogonal test to screen the best extracting parameters and extract chlorogenic acid and galuteolin in Flos lonicerae of the five different production regions under the condition. Then a HPLC method was dopted to determine the content of those samples. Results： Orthogonal experiment demonstrated that the best extracting conditions included solution of 35mL 50% ethanol and extracting time of one hour at 70~C. HPLC method： the mobile phase was 0.5% acetic acid and methanol, gradient elution separation,and detect at wave- length 350nm. A good linearity of chlorogenic acid and galuteolin was within the range of 0.2 - 1.6 ug and 0.2 -2.2 ug respectively, and the linear equations was y = 1 x 106x - 806 7 and y = 4 x 106x - 119 017, with regression coefficient 0. 999 9 and 0. 999 7. The precision, reproducibility, recovery of the method were all proved perfect. The four content of the chlorogenic acid and galuteolin of the five different production regions are higher than the required standard of Chinese Pharmacopoeia.%目的：优化金银花样品中绿原酸和木犀草苷提取工艺，并建立两者含量同时测定的方法。方法：设计三水平四因素的正交试验，筛选最佳提取条件，并以此条件处理不同产地的金银花，用HPLC测定其绿原酸和木犀草苷的含量。结果：最佳提取工艺条件为：用50％的乙醇35mL在70℃下回流提取1h；0．5％冰醋酸和甲醇为流动相，梯度洗脱，检测波长350nm，绿原酸和木犀草苷的线性方程Y=1×106x-8067和Y=4×106X-119017，线性区间为：0．2-1．6ug和O．22-2．21ug，回归系数r值为0。9999和0．9997；精密度、稳定性、回收率试验结果均良好；五个产地的金银花有四个绿原酸和木犀草苷的含量高于药典规定。