一株絮凝剂产生菌TS-1的分离与鉴定

摘要

[ Objective] The purpose was to select out and identify a flocculant producing strain which could produce high active flocculant.[ Method] The strain producing high active flocculant was isolated out and purified through medium culture and the selected strain was identified through observing its culture characters and determining its physiological and biochemical property. [ Result ] Fourteen strains of bacteria with flocculant producing function were isolated from tested soil samples through isolation, purification and preliminary screening using dilution-spread plate method and plate streaking method. Five strains of flocculant producing bacteria showing higher flocculation activty were selected out after second screening and their flocculation rates were higher than 70％; the flocculation activity of one strain among them was still stable after multiple subculturings, its flocculation rate was always higher than 90％ and it was marked as TS-1. TS-1 was encapsulated Grampositive bacillus and there was no lipid in it, such as poly-β- hydroxybutyric acid. TS-1 was Bacillus amyloliquefaciens, so it was named Bacillus TS-1. [ Conclusion] The strain selected out in this experiment could be used in the flocculation and biochemical treatment of wastewater from starch industry.%[目的]筛选鉴定出高絮凝活性的微生物絮凝剂产生菌:[方法丁通过培养基培养分离纯化出高絮凝活性的菌株并通过培养特征观察和生理生化特征测定鉴定筛选出的菌株.[结果]通过用稀释涂布平板法和平板划线法从土壤中分离、纯化和初筛后,从供试土样中共分离到14株具有絮凝性能的细菌,经复筛后得到5株絮凝活性较高(絮凝率>70%)的絮凝剂产生菌,其中1株经多次传代培养后絮凝活性仍很稳定(絮凝率始终>90%),其标记为TS-1.TS-1菌为具有荚膜的革兰氏阳性杆菌,并且菌体内无类脂物质如聚β-羟基丁酸.TS-1为解淀粉芽孢杆菌(Bacillus amyloliquefaciens),故将该菌定名为Bacillus TS-1.[结论]该试验筛选出的菌株可用于淀粉工业废水的絮凝处理和生化处理.