[目的]研究不同杀虫剂对水稻负泥虫(Oulema onzae)体内解毒酶的影响.[方法]以水稻负泥虫为研究对象,分析幼虫在高效氯氰菊酯、阿维菌素和吡虫啉3种杀虫剂处理1、4、9和15 d后,幼虫体内羧酸酯酶及谷胱甘肽转移酶活性变化.[结果]随着取食时间的延长,3种药剂处理的幼虫体内羧酸酯酶活性均较对照增高,且差异明显;而3种药剂处理的谷胱甘肤转移酶活性均低于对照,但差异不明显.[结论]解毒酶的变化在害虫抵抗杀虫剂处理过程中起到指示作用,该研究可为水稻负泥虫抗药性研究奠定基础.%[ Objective ] The aim was to study the effects of different pesticides on the activity of detoxification enzymes in the larvae of O.oryzae. [ Method ] As the model of the larvae of O. oryzae, the change of carboxylesterase and glutathione transferase in larvaes fed with rice leaves treated 1, 4, 9 and 15 days by 3 insecticides, beta-cypermethrin, abamectin and imidacloprid, were analyzed. [ Result ] The results showed that carboxylesterase activity of 3 insecticides treatments in the larvae was higher than the control with the time increased, and the difference among 3 insecticides treatments was significant. While glutathione transferase activity of 3 insecticides treatments in the larvae was lower than the control with the time increased, but the difference among 3 insecticides treatments was no significant. [ Conclusion ] Detoxification enzymes can acted as markers in the process of insecticide pest control. The study can lay a basis for researching of the resistance mechanisms to insecticides of O. oryzae.
展开▼
