水稻蛋白OsOlel在大肠杆菌中的诱导表达

摘要

[目的]在大肠杆菌中诱导表达水稻蛋白OsOlel.[方法]RT-PCR克隆水稻基因OsOlel,构建原核表达载体pGEX-2T-OsOlel-GST,在大肠杆菌B121中诱导表达该蛋白,并采用GST凝胶亲和层析进行纯化.[结果]水稻蛋白OsOlel具有典型的Oleel蛋白家族的保守域,N端1～24位氨基酸为预测的信号肤序列.成功地将水稻蛋白OsOlel在大肠杆菌中进行了诱导表达,并用GST亲和层析进行了纯化.[结论]该研究为以后进一步研究水稻蛋白OsOlel的生理功能奠定了基础.%[ Objective ] The research aimed to induce the expression of rice protein OsOlel in Escherichia coli. [ Method ] RT-PCR was used to clone OsOlel in the rice, and the prokaryotic expression vector pGEX-2T-OsOlel-GST was constructed. The protein was induced expressing in Escherichia coli BL21, and GST gel affinity chromatography was used to purify. [ Result] The rice protein OsOlel had the typical conserved domain of Oleel protein family. The amino acids at 1 - 24 sites in the N terminal were the predicted signal peptide sequence. The rice proteinOsOlel was successfully induced expressing in Escherichia coli, and GST affinity chromatography was used to purify. [ Conclusion] The research laid the foundation for further studying the physiological function of rice protein OsOlel.