[ Objective] Disease analysis and pathogen identification of black spot disease of Chinese cabbage [Alternaria brassicae( Berk. )Sace. ] in Bijie City of Guizhou Province was carried out, so as to raise the corresponding prevention measures. [Method] Pathogen was isolated from diseased leaves using tissue surface isolation method for cultivation, microscopic examination, spore size determination and pathogen identification. [ Result] Microscopic examination showed that the disease was caused by Alternaria sp. , the size of its spores was ( 15.0 - 38.5) μm × (7.5 - 13.5) μm. Combining with the characteristics of host and pathogen, the pathogen caused black spot disease of Chinese cabbage was identified to be Alternaria brassicicola. [ Conclusion] Alternaria brassicicola was the pathogenic fungi of black spot disease of Chinese cabbage in Bijie City of Guizhou Province, and damage caused by the disease could be reduced through agricultural measures and chemical control.%[目的]对贵州省毕节市的白菜黑斑病进行病害分析和病原鉴定,以提出相应的预防措施.[方法]采用表面组织分离法从病叶中分离得到病原菌,并对病原菌进行培养、镜检、孢子大小测定和鉴定.[结果]显微镜镜检的病原菌特征表明,引起白菜黑斑病的病原菌是链格孢菌(Alternaria sp.),病原菌的孢子大小为(15.0～38.5)μm×(7.5～13.5)μm,结合寄主和病原特征,引起白莱黑斑病的病原菌被鉴定为芸薹生链格孢(Alternaria brassicicola).[结论]芸薹生链格孢为贵州省毕节市当前白菜黑斑病的病原菌,可以通过农业措施和药剂预防来减少该病的危害.
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