催乳素释放肽在青年摩杂一代水牛下丘脑-垂体-卵巢轴的免疫组化定位

摘要

[目的]探索催乳素释放肽(PrRP)在青年摩杂一代水牛生殖调控和泌乳中的作用.[方法]应用石蜡切片、HE染色、Nissl染色、免疫组化染色,对5头雌性的青年摩杂一代水牛下丘脑-垂体-卵巢轴上PrRP的分布进行定位,并与IEICA图像分析系统和SPSS13.0统计分析软件相结合进行统计.[结果]在下丘脑内,PrRP免疫反应阳性神经元主要分布在视上核,在后核分布最少;PrRP免疫反应阳性纤维在室旁核高度密集,在弓状核、乳头体核密集,在视前核、前核、视交叉上核、腹内侧核、腹外侧核、背内侧核、背外侧核只有少量分布,在视上核无免疫反应阳性纤维.在垂体,在腺垂体部只观察到PrRP免疫反应阳性细胞,无免疫反应阳性纤维;在神经垂体部,有大量的免疫反应阳性纤维,无免疫反应阳性细胞.在卵巢的卵泡和间质中未见PrRP免疫反应阳性产物,仅在黄体的粒性细胞中发现PrRP免疫反应阳性产物.[结论]PrRP广泛分布在青年摩杂一代水牛下丘脑-垂体-卵巢轴上的各个环节.%[Objective] The function of reproductive adjustment and milking role of the prolactin releasing peptide( PrRP) of young Murrah hybrid buffalo F, was searched. [Method] The distribution of the PrRP in the hypothalamus-pituitary-ovary of five female F,s was lactated through the method of paraffin coated section,HE stain,Nissl stain and immunohistochemical stain,and combined with the LEICA image analysis system and the analysis of statistics software SPSS13.0. F Result] The results showed that the PrRP immunoreactive positive neurons in the hypothalamus were mainly located in anterior nucleus(AN) ,but few,in pasterior nucleus(PN). The high density of PrRP immunoreactive positive fibers was observed in paraventricular nucleus(PVN); the density,in arcuatus nucleus(AN) and mamillarybody nucleus(MBN); and few,in medisal preoptic nucleus ( MPN), AN, supra-chiasmatic nucleus ( SCN ), ventromedialis nucleus ( VMN ) , ventrolateral nucleus ( VLN), dorsomedialis nucleus ( DMN) and dorsallateral nucleus (DLN). No PrRP immunoreactive positive fiber was found in supra-opticus nucleus(SON). In the pituitary, the PrRP immunoreactive positive cells could only be observed in adenohypophysial,but there was no PrRP immunoreactive positive fiber; there were a large number of PrRP immunoreactive positive fibers in neurohypophysis, but no PrRP immunoreactive positive cell. No PrRP immunoreactive positive product was found in the follicle and the mesenchyme of ovary,only few PRrP was found in corpus luteum. [Conclusion] The PrRP was widely distributed in hypothalamus-pituitary-ovary of the young Murrah hybrid buffalo F1.