[Objective]This study aimed to establish an efficient process for separation of phycoerythrin by using Q Sepharose Fast Flow resin and verify its feasibility.[Method]Elution gradient, loading volume and flow rate were optimized to determine the optimal separation condi tion, under which the scale up process was verified.[Result]The optimal condition for separation of phycoerythrin by using Q Sepharose Fast Flow resin was investigated: 30 ml of laver extract was loaded to a Q Sepharose Fast Flow column with a bed volume of 8 ml; subsequently, the column was eiuted under a stepwise gradient elution of 0 ?. 10 -0.35 - 1.00 mol/L NaCI solution ( pH 6.0) at a constant Cow rate of 1 ml/min; the elution peak under 0. 35 mol/L NaCI solution was collected, and the recovery rate and purity coefficient (Ai565/A380 ) of phycoery thrin were delenmined as 44. 3 and 1. 15, respectively. Based on the established process, 75 ml of phycoerythrin extract was loaded to a Q Sepharose Fast Flow column with bed volume of 20 ml for separation, while no significant variation was observed in separation result.[Conclu sion]Phycoerythrin can be well separated from laver extract by Q Sepharose Fast Flow resin and the process is feasible for scale-up.%[目的]对激光诱变黄皮洋葱须根的生物学效应进行研究.[方法]采用CO2激光和He-Ne激光对黄皮洋葱2个品种的湿种子进行辐射,通过随机区组设计,利用生物统计学和生理生化的方法,从个体及生化水平上考查激光诱变黄皮洋葱L1代须根的生物学效应.[结果]经激光诱变处理的黄皮洋葱在须根长度、数目、须根重以及根系活力等方面,表现为不同处理引起的生物学效应差异显著,其中,激光种类不同引起须根长度的变异达到5％显著差异；洋葱品种不同,激光辐照引起的须根变异明显,但各处理间差异均未达到5％显著水平；激光剂量不同,引起须根数的变异达5％显著水平；激光辐射对洋葱根系活力表现为刺激效应.[结论]该研究结果为激光诱变黄皮洋葱育种提供了参考依据.
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