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短葶飞蓬种子活力测定方法的初步研究

         

摘要

[目的]研究短葶飞蓬种子活力的测定方法.[方法]比较种子数目、培养温度对种子发芽的影响,并采用TTC染色测定种子活力.[结果]测得种子出苗率为15.37%,与培养第6天的种子发芽数目比例接近;100和700粒种子发芽试验的重复间发芽率差异较大,300和500粒种子发芽试验的发芽率差异较小;经TTC染色后,短葶飞蓬种子呈现不同类型,可将全部着色和下部着色的种子认定为具有活力的种子,其比例为15.00%,该数值与发芽势和出苗率接近,试验误差较小.[结论]该方法准确可靠,可用于初步测定短葶飞蓬的种子活力.%[Objective] To research the detection methods for seeds vigor of Erigeron breviscapu. [Method] Effects of seed number and cultivation temperature on the germination rate were detected; TTC staining was used to detect the seed vigor. [Result] The germination rate at the 6th day was 15.37% , which was close to the emergence rate of seeds. Germination rate varied greatly in germination tests of 100 and 700 seeds; but there are relatively few differences in the germination tests of 300 and 500 seeds. After TTC staining, E. breviscapu showed different characteristics. The overall colored seeds and bottom colored seeds could be regarded as vigorous seeds, the proportion of which was 15. 00%. This value was close to the germination potential and germination rate. Thus, the experimental error was small. [Conclusion] This method was accurate and reliable and could be used for the preliminarily detection of seed vigor of E. breviscapu.

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