生物农药与化学农药复配对桃枝枯病菌的毒力

摘要

Objective] To screen the effective biopesticides and their mixtures with fungicides against the pathogen Phomopsis amygdali of peach shoot blight.[ Method] The virulence of 10 biopesticides against P.amygdali was tested in the laboratory by mycelial growth inhibition method, and then the mixtures of biopesticides with high virulence and prochloraz, carbendazim and diniconazole respectively, were detected for their laboratory toxicities.[Result] Zhongshengmycin had the strongest virulence, with EC50 value of 0.041 9 μg/mL, and shenqinmycin and tetramycin had better antimicrobial effects, with EC50 values of 0.231 4 and 0.655 4 μg/mL, respectively.When shenqinmycin was mixed with the three fungicides in different proportion, the synergistic ratio (SR) values were all greater than 1.5, having increased toxicity effect.When mixed with the three fungicides, zhongshengmycin and tetramycin had mixed ratios for additive and synergistic toxicity effects. [ Conclusion] The study indicates that the biopesticides mixed with chemical fungicides can increase the toxicity to P.amygdali.%[目的]明确生物农药和化学农药复配对桃枝枯病菌的抑制效果。[方法]采用菌丝生长抑制法，测定10种生物药剂对桃枝枯病菌的毒力，将毒力强的生物农药与化学农药咪鲜胺、多菌灵和烯唑醇进行复配，探讨复配剂对桃枝枯病菌的联合毒力效果。[结果]毒力作用最强的生物农药是中生菌素，其次是申嗪霉素和梧宁霉素，其抑制中浓度（EC50）分别为0．0419、0．2314和0．6554μg／mL，与3种化学药剂复配，发现申嗪霉素与3种化学农药复配均达到增效作用，而中生菌素和梧宁霉素与化学农药复配大多配比具有增效作用或相加作用。[结论]生物农药与化学农药复配能增加对桃枝枯病菌的毒力。