木薯主栽品种高效再生体系的建立与优化

摘要

[目的]通过研究建立木薯主栽品种的再生体系,获得更多的优质研究材料.[方法]根据细胞全能性理论,以木薯幼叶、腋芽为材料,优化培养基组分,诱导木薯体细胞发生、分化,器官等发育,培养再生株系.[结果]不同品种材料间的再生能力总体表现由大到小为:GR3、SC205、GR9891、GR911;腋芽诱导体细胞胚性愈伤组织的效果明显优于幼叶;IBA诱导木薯体细胞胚发生效果优于6-BA,且二者间具有互作作用;IBA与NAA激素组合对诱导木薯不定芽生根效果优于单一使用效果.[结论]建立了4个木薯主栽品种(SC205、GR891、GR911和GR3)高效的再生体系,为木薯种质保护、创新利用研究提供技术基础.%[Objective] The aim was to obtain more high-quality research materials by constructed regeneration system of cassava cultivars. [ Method] According to the cell totipotency, with young leaves and axillary buds as materials, we optimized the medium composition, induced the somatic embryogenesis and differentiation, as well as organ development, and cultivated regeneration lines. [ Result] The regeneration a-bilities for four cultivars were in the order of GR3, SC205, GR9891, GR911;the axillary buds were better than young leaves to induce somatic embryonic callus;IBA had much better effect to induce the formation of cassava somatic embryo than 6-BA, and they showed synergistic effect;The combination of IBA and NAA had better effect to induce the rooting of adventitious buds than using only one. [ Conclusion] Efficient re-generation systems for four main cassava cultivars (SC205, GR891, GR911 and GR3) were established, which provided technical basis for protection and innovation of cassava germplasm.