HPLC法检测明绿萌动过程中牡荆素和异牡荆素含量变化规律

摘要

[目的]为更好地利用安徽明绿资源,开展了绿豆萌动试验中牡荆素和异牡荆素的含量变化规律及其高效液相色谱法(HPLC)检测方法研究.[方法]对安徽特产明绿进行萌动40 h,每4 h取样1次,并用HPLC方法对样品中牡荆素、异牡荆素含量进行检测.[结果]萌动过程中牡荆素、异牡荆素基本上处于波动下降趋势,但在萌动8 h时含量最高,分别为0.57、1.02 mg/g,在16 h时含量最低,并且异牡荆素含量是牡荆素含量的2倍左右.[结论]HPLC法检测牡荆素、异牡荆素方法可靠,明绿萌动过程中,8 h时牡荆素、异牡荆素含量较高.%[Objective] In order to make better use of Anhui Mingguang mung bean resources,the vitexin and isovitexin content changes and HPLC detection methods were studied in the experiment of mung bean sprout.[ Method] To sprout Mingguang mung bean for 40 h, and take sample 1 time per 4 h, vitexin and isovitexin content were detected by HPLC method.[ Result] In the process of germination, the content of vitexin and isovitexin basically was in a downward trend, but in the germination of 8 h was the highest, respectively 0.57 and 1.02 mg/g, at the time of 16 h was the lowest, and the isovitexin basically was about 2 times the contents of vitexin.[ Conclusion] HPLC method was used to detect Vitexin, isovitexin, the method is reliable, in the process of germinating mung bean, Vitexin and isovitexin were higher at 8 h.