百合组培苗生根培养基配方的筛选

摘要

[Objective]To improve the quality of lily tissue cultured seedling and the rate of transplanting survival,and to screen the appropriate rooting medium formula.[Method]Taking lily tissue cultured seedling as test material, the effects of different basic mediums, different concentrations of IBA and activated carbon on the rooting of lily tissue cultured seedling were studied and compared.[Result] The 1/2 MS was chosen as the best basic medium,the formation of root was accelerated after adding IBA and activated carbon, the best concentration of IBA was 0.10 mg/L, the best amount of activated carbon was 0.2 g/L.[Conclusion]The ideal rooting medium of lily tissue cultured seedling is 1/2MS + IBA 0.10 mg/L + activated carbon 0.2 g/L,average rooting rate is 100%,the roots grow thick and strong under the formula.%[目的]提高百合组培苗的质量和移栽成活率,筛选适宜的生根培养基配方.[方法]以百合组培苗为试验材料,比较不同配比基本培养基、不同浓度IBA和活性炭对百合组培苗生根的影响.[结果]基本培养基选用1/2MS为最好,添加 IBA和活性炭后均加速根的形成,IBA添加的最佳浓度为0.10 mg/L,活性炭加入量以0.2 g/L为最佳.[结论]百合组培苗理想的生根培养基为1/2MS+IBA 0.10 mg/L+活性炭0.2 g/L,此配方下平均生根率为100%,且根密而壮.