骤冷对虎纹蛙和中华大蟾蜍不同组织中MDA含量和SOD活性的影响及比较

摘要

[目的]探讨骤冷对虎纹蛙(Hoplobatrachus chinensis)和中华大蟾蜍(Bufo gargarizans)不同组织中丙二醛(Malonaldehy,MDA)含量和超氧化物歧化酶(Superoxide dismutase,SOD)活性的影响.[方法]采用低温恒温培养箱进行冷暴露试验,研究了骤冷6 h分别对虎纹蛙和中华大蟾蜍肝脏、肠和肾脏MDA含量及SOD活性的影响,并比较骤冷6 h对虎纹蛙和中华大蟾蜍肝脏、肠和肾脏MDA含量及SOD活性的影响.[结果]骤冷刺激后虎纹蛙肝脏、肠和肾脏中MDA含量不变,SOD活性均降低.骤冷刺激后中华大蟾蜍肝脏中MDA含量不变,肠和肾脏中MDA含量增加;肝脏中SOD活性下降,肠组织中SOD活性增大,肾脏中SOD活性不变.对比骤冷对虎纹蛙和中华大蟾蜍肝脏、肠和肾脏中MDA含量和SOD活性的影响,发现中华大蟾蜍的肝脏、肠和肾脏中MDA的相对含量和SOD的相对活性均高于虎纹蛙的肝脏、肠和肾脏中MDA的相对含量和SOD的相对活性.[结论]在遇到骤冷刺激时虎纹蛙的氧化压力和抗氧化能力均低于中华大蟾蜍.%[Objective] To explore the effects of shock chilling on malonaldehyhy (MDA) content and superoxide dismutase (SOD) activity in different tissues of Hoplobatrachus chinensis and Bufo gargarizans.[Method] Cold exposure experiment was conducted by using low temperature incubators to study the effects of shock chilling 6 h on content of MDA and the activity of SOD in the liver,intestine and kidney of H.chinensis and B.gargarizans.And the effects of shock chilling in 6 h on the MDA and the activity of SOD in the liver,intestine and kidney between H.chinensis and B.gargarizans were compared.[Result]After shock chilling,MDA content in the liver,intestine and kidney of H.chinensis had no change,and SOD activity decreased.After shock chilling,MDA content in the liver of B.gargarizans had no change,but that in the intestine and kidney in-creased;SOD activity in the liver decreased,SOD activity in the intestine increased,and SOD activity in the kidney of B.gargarizans remained unchanged.The effects of shock chilling on MDA content and SOD activity in the liver,intestine and kidney between H.chinensis and B.gargari-zans were compared,it was found that the relative content of MDA and relative activity of SOD in the liver,intestine and kidney of B.gargarizans were higher than that in H.chinensis.[Conclusion] Under shock chilling,the oxidative stress and antioxidant capacity of H.chinensis were lower than those in B.gargarizans.