蛹虫草摇瓶菌种培养基的优化

摘要

[目的]优化蛹虫草摇瓶菌种培养基.[方法]以菌丝体干质量为指标,首先采用单因素试验筛选适于菌丝体生长的最佳碳氮源,再以正交试验优化碳源与氮源以及无机盐与VB1的最佳配比.[结果]蛹虫草优化的摇瓶培养基配方为红薯50g/L、可溶性淀粉10 g/L、牛肉膏10 g/L、酵母膏10 g/L、KH2PO4 1.5 g/L、MgSO4·7H2O 1.0 g/L、VB10.10 g/L,pH自然,利用该配方菌丝干质量可达36.33 g/L.[结论]优化的摇瓶培养基可为后续研究和生产提供基础数据.%[Objective]To optimize culture medium for shaking flask of Cordyceps militaris.[Method] Using mycelium dry quality as the index.First,the best carbon and nitrogen source suitable for mycelia growth was selected by single factor test.Then,the optimum ratio of carbon source and nitrogen source,inorganic salt and VB1 was optimized by orthogonal experiment.[Result] The optimum shaking flask medium for Cordyceps militaris was as follow:sweet potato 50 g/L,soluble starch 10 g/L,beef paste 10 g/L,yeast extract 10 g/L,KH2PO4 1.5 g/L,MgSO4 · 7H2O 1.0 g/L,VB1 0.10 g/L,pH natural.The dry weight of mycelia produced by this formula can reach 36.33 g/L.[Conclusion]The optimizing culture medium for shaking flask of Cordyceps militaris can provide basic data for subsequent research and production.